eShop » Process Filtration » Ultrafiltration Cassettes » Consieve™ MET Microfiltration Cassettes PVDF Membrane Process, Reusable
TFF Cassette structure details
tangential flow device principle
Tangential Flow Filtration vs Normal Flow Filtration
TFF Cassette structure details
tangential flow device principle
Tangential Flow Filtration vs Normal Flow Filtration

Consieve™ MET Microfiltration Cassettes PVDF Membrane Process, Reusable

In stock
Part No. MFDFLVM10250P
Filtration Area
MWCO
Screen Type
Loading locations...
*Not for medical use

Consieve™ MET microfiltration TFF cassettes are often used in the clarification process of the supernatant after centrifugation of fermentation broth or lysate. It has the characteristics of high process throughput, large filtration loading capacity, good clarification effect, and easy cleaning. The relaively open suspended flow channel is compatible with filter fluids with a certain solid content and higher viscosity.

The cassettes should be used with Cobetter Stainless Steel TFF Cassette Holder:

EFA 0.02m² cassettes use with Ultrafiltration Cassette Holders (0.01m²)

EFA 0.02m² or 0.1m² cassettes use with Ultrafiltration Cassette Holders (0.11m²)

EFA 0.5m²or 2.5m²cassettes use with Ultrafiltration Cassette Holders (0.5m²)

Features

  • Very hydrophilic
  • High throughput
  • High loading capacity
  • Good anti-pollution ability

Applications

  • Clarification process of the supernatant after centrifugation of fermentation broth or lysate

Quality Standards

  • ISO 9001: 2015 Quality Management System
  • Manufactured for use in conformance with cGMP
  • Each product is integrity tested prior to release
  • COA/COQ provided with shipment
  • Validation documents are available upon request
  • TSE/BSE certificate available
Physical Properties Filter Format Consieve™ MET TFF Cassettes
Scale Commercial Manufacturing
Filtration Area 2.5 m²
Flow Channel V Type - Suspended Screen
MWCO 0.1/ 0.2/ 0.45/ 0.65 μm
Materials Membrane Material Polyvinylidene difluoride (PVDF)
Screen Material Polypropylene (PP)
Gasket Material Silicone
Encapsulant Material Silicone
Operating Conditions pH Stability 2.0-11.0
Max. Operating Pressure 4 bar
Max. Operating Temperature 50 °C
Regulatory Compliance Compliance USP <788> Particulate Matter
USP <87> Cytotoxicity
USP <88> Biological Reactivity
FDA Indirect Food Additives, 21 CFR 177-182
ADCF Materials
Bacterial Endotoxin Endotoxin Limit of WFI System<0.25 EU/mL with the Limulus Amebocyte Lysate (LAL), USP <85>
Usage Protocols Reusable Yes
Pre-use Treatment Shipped wet in an aqueous solution containing 0.1M~0.2M NaOH and 20% glycerin. Flush the cassette well with water prior to use.
Sterility Non-sterile
Package Pack Size Pack of 1
Packaging Individually packed in a double PE bag

 
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What is tangential flow filtration (TFF)?

Tangential flow filtration (TFF) is a method in which the feed flows along the membrane surface, generating tangential shear to reduce membrane fouling and clogging. TFF is commonly applied as ultrafiltration (1–1000 kDa) or microfiltration (0.1–0.65 µm).

How to choose the appropriate ultrafiltration membranes?

For most ultrafiltration and dialysis applications, both PES and RC membranes are suitable choices.

PES membranes can meet a wide range of application needs for most protein and small-molecule products, such as antibodies, APIs, and vaccines.

RC membranes should be considered when preparing high-concentration formulations, applications containing organic solvents (e.g., ADC products), or concentration/exchange of linear molecules including oligonucleotides and peptides.

For new target molecules, it is recommended to test both membrane types during standard sample handling and process optimization.

How to select the appropriate cut-off for ultrafiltration cassettes?

Choose the membrane cut-off based on the size or molecular weight of the substance to be separated or retained:

Antibody proteins: 30 kDa or 50 kDa
Viral or vaccine products: 100–1000 kDa
For API products, an appropriate molecular weight cut-off (MWCO) can be selected from the range of 1 kDa, 2 kDa, 3 kDa, 5 kDa, 8 kDa, and 10 kDa according to the process objectives.

If the molecular weight of the target substance is unknown, preliminary testing can be performed using ultrafiltration centrifugal devices with different cut-offs to identify the most suitable molecular weight retention.

What materials and cut-offs are available for Cobetter TFF cassettes?

Ultrafiltration cassettes are available in PES and RC membranes, with cut-offs ranging from 1–1000 kDa.

Microfiltration cassettes are available in PES and PVDF membranes, with pore sizes ranging from 0.1–0.65 µm.

Can TFF cassettes be reused?

Yes. TFF cassettes can be reused by selecting appropriate cleaning methods. However, due to changes such as declining membrane flux and increased permeation, the cassettes should be replaced periodically as needed.

How should TFF cassettes be cleaned?

They can be cleaned by circulating 0.1-0.5 M sodium hydroxide (NaOH) solution. If cleaning effectiveness is insufficient, adjustments can be made by either of the following methods:

  • Increasing the cleaning solution temperature (<45 °C)
  • Adding sodium hypochlorite (NaClO, 500 ppm)
  • Extending the cleaning time

For difficult-to-clean feed components, alternative cleaning solutions such as acidic solutions, alcohols, or surfactants may also be used.

Are Cobetter TFF cassettes compatible with hardware from other companies?

Yes. They can be installed in holders from Merck, Pall, and similar manufacturers. But it is necessary to ensure proper torque and perform integrity testing.

For Sartorius holders, a specific adapter plate is required for proper installation.