-
-20%0.5mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane 24/pk
Membrane RCMWCO 2/3/10/30/50/100/300 kDaFiltration Area 0.6cm²Package 24/pk -
-20%2mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane 15/pk
Membrane RCMWCO 2/3/10/30/50/100 kDaFiltration Area 1.4cm²Package 15/pk -
-20%4mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane 15/pk
Membrane RCMWCO 2/3/10/30/50/100/300 kDaFiltration Area 3.4cm²Package 15/pk -
-20%15mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane
Membrane RCMWCO 2/3/10/30/50/100/300 kDaFiltration Area 7.4cm²Package 4/pk or 24/pk
Collection:
Centrifugal Filters
Cobetter Briscale™ centrifugal filters are used for the concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. They are made of low protein binding Regenerated Cellulose membranes, which feature high protein recovery. The unique design of the ultrafiltration devices offers fast and reliable concentrations of up to 80-fold. The vertical membrane design reduces concentration polarization and facilitates the spin times as few as 15-30 minutes.
Available MWCO: 2kDa, 3kDa, 10kDa, 30kDa, 50kDa, 100kDa, 300kDa.
Overview
What is a Centrifugal Filter?
Centrifugal filters (or ultrafiltration devices) are essential tools in labs for concentrating proteins, purifying nucleic acids, or desalting samples. A centrifugal filter uses a ultrafiltration membrane with a specific molecular weight cut-off (MWCO) to separate molecules. During centrifugation, smaller molecules pass through the membrane, while larger ones are retained.
Features
- High product recovery --- 50 to 70-fold concentration
- Quick processing time --- The general concentration time is 10-60 minutes.
- High recovery rate ---85% protein recovery. The RC membrane have extremely low protein adsorption.
- A variety of MWCO --- 2kDa, 3kDa, 10kDa, 30kDa, 50kDa, 100kDa and 300kDa.
- Color-coded cap --- for easy detection and user-friendly
- Non-sterile and single-use --- Can be disinfected with a 0.1-0.5 N NaOH solution or 70% ethanol
Cobetter Briscale™ centrifugal filters are available in 4 different volumes:
- Briscale™ UF 15mL centrifugal filters
- Briscale™ UF 4mL centrifugal filters
- Briscale™ UF 2mL centrifugal filters
- Briscale™ UF 0.5mL centrifugal filters
Applications
- Protein Concentration: Quick concentration and purification of proteins such as antigens, antibodies, enzymes and biological samples such as nucleic acids and microorganisms.
- Buffer Exchange: Replace salts or detergents without dialysis.
- DNA/RNA Cleanup: Remove primers or dyes after PCR.
- Virus Purification: Isolate viral particles from culture media.
How to Choose a Centrifugal Filter?
- Two main properties should be considered in selection: MWCO and Working Volume.
- To choose which type to use, determine the molecular weight (MW) of your macromolecule of interest, and select an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule. (e.g., for a 35 kDa protein, use a 10kDa filter).
- Then add your sample to the filter, taking care not to overfill the device. Typically, samples should not exceed 2/3 of the filter's maximum volume capacity.
Using a centrifugal filter save big time in protein sample preparation. And the success of your research test depends on choosing the right MWCO and protocol. Match the centrifugal filter to your molecule size, and optimize centrifugation speed for excellent performance.
Related Articles:
How to Use Centrifugal Filter Units?
How to Select the Correct MWCO for DNA, RNA and Protein Purification and Concentration
Video
Download
- Cobetter_Laboratory_Consumables__Catalog__20250301 Download
FAQ
