-
-10%0.2μm PVDF Transfer Membranes
Membrane Hydrophobic PVDFPore size 0.2μmPackage a roll or 50 pcs/pk -
-10%
0.45μm PVDF Transfer Membranes
Membrane Hydrophobic PVDFPore size 0.45μmPackage a roll or 50 pcs/pk -
-98%Free Sample - PVDF Transfer Membrane 13.5cm*15cm
Membrane Hydrophobic PVDFPore size 0.2/0.45μmPackage 1pcs/pk
Collection:
PVDF Transfer Membrane
Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The PVDF membrane is hydrophobic and needs to be prewetted by methanol. We offer pre-cut membrane sheets as well as coils. And available in 0.45μm for most Western blotting application and 0.2μm for immunoblotting of low MW proteins <20kDa.
Overview
Western Blotting is a method to analyze and quantify proteins. The protein is separated by gel electrophoresis to a solid phase support (PVDF membrane). Antibodies can generate antigen-antibody immune reactions with the target proteins attached to the PVDF membrane. The western blotting technology is to identify and semi quantitatively analyze a specific protein.
Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The PVDF membrane is hydrophobic and needs to be prewetted by methanol. We offer pre-cut membrane sheets as well as coils. And available in 0.45μm for most Western blotting application and 0.2μm for immunoblotting of low MW proteins <20kDa.
Procedure of Western Blot
The western blotting protocol can be roughly divided into the following 7 steps: cell culture, protein extraction, concentration and exchange, SDS-PAGE electrophoresis, membrane transfer, blocking and antibody incubation, and protein detection.
Selection Guide of Cobetter PVDF Transfer Membrane
Precautions
- Prevent bubbles from entering into the filter paper, gel or membrane
- For samples contain small peptides, the equilibrium of the gel in the transfer buffer should be limited into 10 minutes
- When the target protein is less than or is 20kDa, 0.2μm PVDF transfer membrane is preferred
- The PVDF transfer membrane is only used for laboratory research, and should not be used for clinical diagnosis or treatment
Video
Download
FAQ
