PVDF Membrane Western Blot

Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The PVDF membrane is hydrophobic and needs to be prewetted by methanol. We offer pre-cut membrane sheets as well as coils. And available in 0.45μm for most Western blotting application and 0.2μm for immunoblotting of low MW proteins <20kDa.

PVDF Membrane Western Blot

Overview

Western Blotting is a method to analyze and quantify proteins. The protein is separated by gel electrophoresis to a solid phase support (PVDF membrane). Antibodies can generate antigen-antibody immune reactions with the target proteins attached to the PVDF membrane. The western blotting technology is to identify and semi quantitatively analyze a specific protein.

Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The PVDF membrane is hydrophobic and needs to be prewetted by methanol. We offer pre-cut membrane sheets as well as coils. And available in 0.45μm for most Western blotting application and 0.2μm for immunoblotting of low MW proteins <20kDa.

Procedure of Western Blot

The western blotting protocol can be roughly divided into the following 6 steps: SDS-PAGE electrophoresis, membrane transfer, blocking and antibody incubation, and protein detection.

PVDF Transfer Membrane Activation Guide

Precautions

Prevent bubbles from entering into the filter paper, gel or membrane
For samples containing small peptides, the equilibrium time of the gel in the transfer buffer should be limited to 10 minutes
When the target protein is less than or equal to 15 kDa, 0.2μm PVDF transfer membrane can be used to obtain the maximum retention.
The PVDF transfer membrane is only used for scientific research by professionals, and should not be used for clinical diagnosis or treatment, food or medicine.
For your safety and health, please ware lab clothes and disposable gloves.

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