0.45μm PVDF Transfer Membranes for Western Blot
Regular price
$193.50 USD
Regular price
$215.00 USD
Sale price
$193.50 USD
(-10%)
Couldn't load pickup availability
*Bulk Order? Request a Quote for Big Savings Now
*Ships out within 3-7 Business Days
*Read our Shipping Policy and Refund Policy
*Not for medical use
Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The 0.45μm is suitable for most Western blotting applications and 0.2μm for immunoblotting of low MW proteins <20kDa.
Specifications
| Membrane | Hydrophobic PVDF | ||
| Pore Size |
|
||
| Thickness |
|
||
| Protein Binding Capacity (BSA) |
|
||
| Dimension |
275mm x 3.75m 84mm*70mm 150mm*150mm 200mm*200mm |
||
| Main Binding Mechanisms | Electrostatic, Hydrophobic | ||
| Pre-activation | Requires ethanol (methanol) | ||
| Tolerance | Resistant to most organic solvents, acids and weak bases | ||
| Compatibility | Compatible with commonly used transfer conditions and detection methods (such as staining, chemiluminescence, radiolabeling, etc.) | ||
| Storage | Store the membrane flat at ambient temperature away from chemical vapors. Some solvent vapors may dissolve parts of the membrane, thereby disrupting the pore structure. | ||
| Package | 1 roll/pk or 50pcs/pk |
Features
- Smooth and flat surface, won't curl or fracture when cut
- High mechanical strength, easy to be stripped and reprobed multiple times
- Uniform pore size, high protein mobility, clear and neat bands
- High sensitivity and low background, guarantee the success rate of low abundance protein detection
Applications
- Protein transfer
- Blot detection
- Adsorption analysis
PVDF Transfer Membrane Activation Guide
Precautions
- Prevent bubbles from entering into the filter paper, gel or membrane
- For samples containing small peptides, the equilibrium time of the gel in the transfer buffer should be limited to 10 minutes
- When the target protein is less than or equal to 15 kDa, 0.2μm PVDF transfer membrane can be used to obtain the maximum retention.
- The PVDF transfer membrane is only used for scientific research by professionals, and should not be used for clinical diagnosis or treatment, food or medicine.
- For your safety and health, please ware lab clothes and disposable gloves.