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0.45µm PVDF Transfer Membranes for Western Blot

In stock
Part No. 2690H-R2703
$193.50 USD
$215.00 USD $193.50 USD (-10%)
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*Not for medical use

Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The 0.45μm is suitable for most Western blotting applications and 0.2μm for immunoblotting of low MW proteins <20kDa.

Specifications

Membrane Hydrophobic PVDF
Pore Size
0.2 µm 0.45 µm
Thickness
130 µm 125 µm
Protein Binding Capacity (BSA)
300 µg/cm² 135 µg/cm²
Dimension

275mm x 3.75m

84mm*70mm

150mm*150mm

200mm*200mm
Main Binding Mechanisms Electrostatic, Hydrophobic
Pre-activation Requires ethanol (methanol)
Tolerance Resistant to most organic solvents, acids and weak bases
Compatibility Compatible with commonly used transfer conditions and detection methods (such as staining, chemiluminescence, radiolabeling, etc.)
Storage Store the membrane flat at ambient temperature away from chemical vapors. Some solvent vapors may dissolve parts of the membrane, thereby disrupting the pore structure.
Package 1 roll/pk or 50pcs/pk

Features

  • Smooth and flat surface, won't curl or fracture when cut
  • High mechanical strength, easy to be stripped and reprobed multiple times
  • Uniform pore size, high protein mobility, clear and neat bands
  • High sensitivity and low background, guarantee the success rate of low abundance protein detection

Applications

  • Protein transfer
  • Blot detection
  • Adsorption analysis

PVDF Transfer Membrane Performance

Precautions 

  • Prevent bubbles from entering into the filter paper, gel or membrane
  • For samples containing small peptides, the equilibrium time of the gel in the transfer buffer should be limited to 10 minutes
  • When the target protein is less than or equal to 15 kDa, 0.2μm PVDF transfer membrane can be used to obtain the maximum retention.
  • The PVDF transfer membrane is only used for scientific research by professionals, and should not be used for clinical diagnosis or treatment, food or medicine.
  • For your safety and health, please ware lab clothes and disposable gloves.

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