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TFF Cassette Holder 0.5m²
TFF Cassette Holder 0.5m²

Consieve Ultrafiltration Cassettes Holder 0.5m², with 3 Valves and 2 Gauges

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Part No. H-MB050T25-LP
$3,572.00 USD
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*Not for medical use

Due to the forces required to drive a tangential flow filtration process, a robust holder is needed to provide support against internal pressures. Further, the holder and gaskets can provide the liquid distribution of the feed, retentate, and permeate lines.

Cobetter TFF Cassette Holder (0.5m²) is available for use with 0.5m² or 2.5m² cassettes, with a maximum cassette capacity of 5m². Suitable for applications with throughput volumes more than 50 L.

Applications

Process development and pilot-scale or large-scale production in biopharmaceutical and pharmaceutical ultrafiltration.
Connections Connectors T20
Materials Filter Plate Material 316L stainless steel
O-rings Silicone
Physical Properties Applications Pilot Production, Large Scale Production
Surface Treatment Polishing
Pressure Withstand 0.6 MPa
Cassette Capacity 0.46m² or 2.33m² cassettes
Throughput >50 L
Dimensions 28 x 9 x 25 cm
Weight 24 kg
Accessories 3 PP Manual Extrusion Valves

Brand: Fujikin

Connector: TC25

Pipe Diameter: 3/4 inch

PTFE+EPDM diaphragm
2 Pressure Gauges

TC Connection

Monitoring Range: 0-6 bar
Package Packaging Individually packed
Pack Size 1 pc/pk

 
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What is tangential flow filtration (TFF)?

Tangential flow filtration (TFF) is a method in which the feed flows along the membrane surface, generating tangential shear to reduce membrane fouling and clogging. TFF is commonly applied as ultrafiltration (1–1000 kDa) or microfiltration (0.1–0.65 µm).

How to choose the appropriate ultrafiltration membranes?

For most ultrafiltration and dialysis applications, both PES and RC membranes are suitable choices.

PES membranes can meet a wide range of application needs for most protein and small-molecule products, such as antibodies, APIs, and vaccines.

RC membranes should be considered when preparing high-concentration formulations, applications containing organic solvents (e.g., ADC products), or concentration/exchange of linear molecules including oligonucleotides and peptides.

For new target molecules, it is recommended to test both membrane types during standard sample handling and process optimization.

How to select the appropriate cut-off for ultrafiltration cassettes?

Choose the membrane cut-off based on the size or molecular weight of the substance to be separated or retained:

Antibody proteins: 30 kDa or 50 kDa
Viral or vaccine products: 100–1000 kDa
For API products, an appropriate molecular weight cut-off (MWCO) can be selected from the range of 1 kDa, 2 kDa, 3 kDa, 5 kDa, 8 kDa, and 10 kDa according to the process objectives.

If the molecular weight of the target substance is unknown, preliminary testing can be performed using ultrafiltration centrifugal devices with different cut-offs to identify the most suitable molecular weight retention.

What materials and cut-offs are available for Cobetter TFF cassettes?

Ultrafiltration cassettes are available in PES and RC membranes, with cut-offs ranging from 1–1000 kDa.

Microfiltration cassettes are available in PES and PVDF membranes, with pore sizes ranging from 0.1–0.65 µm.

Can TFF cassettes be reused?

Yes. TFF cassettes can be reused by selecting appropriate cleaning methods. However, due to changes such as declining membrane flux and increased permeation, the cassettes should be replaced periodically as needed.

How should TFF cassettes be cleaned?

They can be cleaned by circulating 0.1-0.5 M sodium hydroxide (NaOH) solution. If cleaning effectiveness is insufficient, adjustments can be made by either of the following methods:

  • Increasing the cleaning solution temperature (<45 °C)
  • Adding sodium hypochlorite (NaClO, 500 ppm)
  • Extending the cleaning time

For difficult-to-clean feed components, alternative cleaning solutions such as acidic solutions, alcohols, or surfactants may also be used.

Are Cobetter TFF cassettes compatible with hardware from other companies?

Yes. They can be installed in holders from Merck, Pall, and similar manufacturers. But it is necessary to ensure proper torque and perform integrity testing.

For Sartorius holders, a specific adapter plate is required for proper installation.