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Consieve™ 2 UET Ultrafiltration Cassettes PES Membrane Process, with PU Encapsulant, Reusable

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Part No. UF2ME001SC25P
Filtration Area
MWCO
Screen Type
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*Not for medical use

Consieve™ 2 UET tangential flow filtration cassette is made of asymmetric mPES (modified Polyethersulfone) membrane, which provides high flow rate, low protein bindings and wide pH compatibility. They are available in a wide range of molecular weight cut-offs from 1 kDa to 1000 kDa.The cassette is easy to clean, install and has a high retention efficiency, with low working volume and high efficiency which ensures product yields.

Consieve™ 2 series ultrafiltration cassettes represent an enhanced iteration of Cobetter Consieve line, incorporating field-proven design optimizations in membrane structure and material selection. Consieve™ 2 UET cassettes employ polyurethane resin encapsulation, significantly increasing structural rigidity and compression resistance, ensuring dimensional stability of flow channels.

The cassettes demonstrate excellent linear scalability from lab-scale development to production-scale manufacturing. With optimized flow path configurations and multiple channel options, users can select the most suitable flow structure based on application requirements to improve process economics.

The cassettes should be used with Cobetter Stainless Steel TFF Cassette Holder:

EFA 0.01m² or 0.02m² cassettes use with Ultrafiltration Cassette Holders (0.01m²)

EFA 0.02m² or 0.11m² cassettes use with Ultrafiltration Cassette Holders (0.11m²)

EFA 0.5m²or 2.5m²cassettes use with Ultrafiltration Cassette Holders (0.5m²)

Features

  • High flow rate
  • Low protein binding
  • High product yield
  • No glue used, resulting in low extractables
  • pH range: 1-14

Applications

  • Concentration and desalting of proteins, peptides or nucleic acids
  • Buffer exchange
  • Fractionation and purification
  • Sample preparation before chromatography
  • Remove pyrogens from water, buffers, and media solutions

Quality Standards

  • ISO 9001: 2015 Quality Management System
  • Manufactured for use in conformance with cGMP
  • Each product is integrity tested prior to release
  • COA/COQ provided with shipment
  • Validation documents are available upon request
  • TSE/BSE certificate available
Physical Properties Filter Format Consieve™ 2 TFF Cassettes
Scale Commercial Manufacturing
Filtration Area 2.5 m²
Flow Channel A Type - Tight Screen
C Type - Standard Coarse Screen
D Type - Coarse Screen
V Type - Suspended Screen
MWCO 1/ 3/ 5/ 8/ 10/ 30/ 50/ 100/ 300/ 500/ 1000 kDa
Materials Membrane Material Polyethersulfone (PES)
Screen Material Polypropylene (PP)
Gasket Material Silicone
Encapsulant Material Polyurethane
Operating Conditions pH Stability 1.0-14.0
Max. Operating Pressure 4 bar
Max. Operating Temperature 50 °C
Regulatory Compliance Compliance USP <788> Particulate Matter
USP <87> Cytotoxicity
USP <88> Biological Reactivity
FDA Indirect Food Additives, 21 CFR 177-182
ADCF Materials
Bacterial Endotoxin Endotoxin Limit of WFI System<0.25 EU/mL with the Limulus Amebocyte Lysate (LAL), USP <85>
Usage Protocols Reusable Yes
Pre-use Treatment Shipped wet in an aqueous solution containing 0.1M~0.2M NaOH and 20% glycerin. Flush the cassette well with water prior to use.
Sterility Non-sterile
Package Pack Size Pack of 1
Packaging Individually packed in a double PE bag


Screen Difference

Screen Type Features Applications Recommended Feed Flow Rate
Tight Screen (A) High shear rate
High mass transfer efficiency
High pressure drop		 Low-concentration, low-viscosity clarified solutions; Recommended protein concentration <100 g/L 4-6 L/min/m²
Standard Coarse Screen (C) Medium shear rate
Medium mass transfer efficiency
Medium pressure drop		 Higher-concentration, higher-viscosity clarified solutions; Recommended protein concentration 100–200 g/L 5-7 L/min/m²
Coarse Screen (D) Low shear rate
Low mass transfer efficiency
Low pressure drop		 High-concentration, high-viscosity, low-turbidity solutions; Recommended protein concentration 200–300 g/L 5-8 L/min/m²
Suspended Screen (V) Very low shear rate
Very low mass transfer efficiency
Very low pressure drop		 Turbid solutions with relatively high solid content 7-10 L/min/m²

 
  • Cobetter_Consieve_2_TFF_Cassettes_and_Systems_260126 Download
What is tangential flow filtration (TFF)?

Tangential flow filtration (TFF) is a method in which the feed flows along the membrane surface, generating tangential shear to reduce membrane fouling and clogging. TFF is commonly applied as ultrafiltration (1–1000 kDa) or microfiltration (0.1–0.65 µm).

How to choose the appropriate ultrafiltration membranes?

For most ultrafiltration and dialysis applications, both PES and RC membranes are suitable choices.

PES membranes can meet a wide range of application needs for most protein and small-molecule products, such as antibodies, APIs, and vaccines.

RC membranes should be considered when preparing high-concentration formulations, applications containing organic solvents (e.g., ADC products), or concentration/exchange of linear molecules including oligonucleotides and peptides.

For new target molecules, it is recommended to test both membrane types during standard sample handling and process optimization.

How to select the appropriate cut-off for ultrafiltration cassettes?

Choose the membrane cut-off based on the size or molecular weight of the substance to be separated or retained:

Antibody proteins: 30 kDa or 50 kDa
Viral or vaccine products: 100–1000 kDa
For API products, an appropriate molecular weight cut-off (MWCO) can be selected from the range of 1 kDa, 2 kDa, 3 kDa, 5 kDa, 8 kDa, and 10 kDa according to the process objectives.

If the molecular weight of the target substance is unknown, preliminary testing can be performed using ultrafiltration centrifugal devices with different cut-offs to identify the most suitable molecular weight retention.

What materials and cut-offs are available for Cobetter TFF cassettes?

Ultrafiltration cassettes are available in PES and RC membranes, with cut-offs ranging from 1–1000 kDa.

Microfiltration cassettes are available in PES and PVDF membranes, with pore sizes ranging from 0.1–0.65 µm.

Can TFF cassettes be reused?

Yes. TFF cassettes can be reused by selecting appropriate cleaning methods. However, due to changes such as declining membrane flux and increased permeation, the cassettes should be replaced periodically as needed.

How should TFF cassettes be cleaned?

They can be cleaned by circulating 0.1-0.5 M sodium hydroxide (NaOH) solution. If cleaning effectiveness is insufficient, adjustments can be made by either of the following methods:

  • Increasing the cleaning solution temperature (<45 °C)
  • Adding sodium hypochlorite (NaClO, 500 ppm)
  • Extending the cleaning time

For difficult-to-clean feed components, alternative cleaning solutions such as acidic solutions, alcohols, or surfactants may also be used.

Are Cobetter TFF cassettes compatible with hardware from other companies?

Yes. They can be installed in holders from Merck, Pall, and similar manufacturers. But it is necessary to ensure proper torque and perform integrity testing.

For Sartorius holders, a specific adapter plate is required for proper installation.