Home » Laboratory » Centrifugal Filters » Briscale 4mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane 15/pk
centrifugal filter structure diagram
centrifugal filter structure diagram

Briscale 4mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane 15/pk

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Part No. ULRC1000040P
$76.50 USD
$85.00 USD $76.50 USD (-10%)
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Cobetter Briscale 4mL centrifugal filters are made of low-binding regenerated cellulose(RC) ultrafiltration membranes, which can achieve a high protein recovery rate >90%, using marker protein Lysozyme(14.4 kDa) at 0.25 mg/mL, centrifuge 60 mins.

Briscale centrifugal filter is ideal for the concentration of proteins and biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. The vertical design and membrane surface area provide fast concentration time and rapid protein concentration for up to 80-fold concentration.

The centrifugal filter unit consists of a lid, a filter device and a centrifuge tube. Use a pipette to collect the concentrate from the filter device. The ultrafiltrate is collected in the centrifuge tube for further analysis.

RC Centrifugal Filters can handle samples up to 4 mL in rotors accepting 15 mL centrifuge tubes. Use in fixed angle 45°rotor(max starting volume of 4mL) or swing bucket rotor(max starting volume of 4mL).

Features

  • RC membrane achieves a high recovery rate of>90%
  • Color-coded cap with different MWCO, volume graduation for easy detection and user-friendly
  • Low-binding regenerated cellulose(RC) ultrafiltration membranes
  • Broad chemical compatibility pH 1-14
  • 80-fold concentration with vertical membranes
  • Translucent housing and volume marker make sample monitoring effortless.

Applications

  • Concentration and purification of nucleic acids, proteins such as antigens, antibodies, enzymes, etc and other biomolecules
  • Desalting, buffer exchange and dialyzing proteins and antibodies for sample preparation
  • Concentration of dilute or pre-purified proteins from chromatography
  • Remove primers, adapters or molecular tags from the reaction mixture
  • Purification of macromolecules in tissue culture extracts or cell lysates, and protein removal prior to HPLC and mass spectrometry.

Centrifugal Filter Units Structure & Adapted Equipment

Cobetter ultrafiltration centrifugal filter units consist of a lid, a filter unit, and a centrifuge tube.

It requires a rotor or rotor adapter compatible with 15mL (for 4mL centrifugal filter) or 50mL (for 15mL centrifugal filter) conical bottom centrifuge tubes. Both the swinging bucket rotor and the fixed-angle rotor must meet this requirement. Learn more about how to use a centrifugal filter.

Protein Recovery Rate

The RC membrane and smooth inner wall design have extremely low protein adsorption. Can achieve a recovery rate of more than 90%.
Physical Properties Intended Use for Research Use Only
Filter Format BriScale™ Centrifugal Filter
Filtration Area 3.4 cm²
Dimentions Length x Tube diameter: 124.5 * 16.9 mm
MWCO 2/ 3/ 10/ 30/ 50/ 100/ 300 kDa
Materials Membrane Material Regenerated Cellulose (RC)
Lid HDPE
Ultrafiltration Device GPPS
Centrifuge Tube PP
Operating Conditions Maximum Sample Volume Swing Bucket Rotor: 4 mL
Fixed Angle Rotor (45°): 4 mL
Minimum Retentate Volume 80-200 uL
Recommended Speed 4,000 - 5,000 g
Sterility Delivery Condition Non sterile
Sterilization Method Disposable
Package Pack Size Pack of 15
Packaging Individually packed in a double PE bag

 

How to choose Cobetter Centrifugal Filters?

To choose which type to use, determine the molecular weight (MW) of your macromolecule of interest, and select an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule. Read more details about how to choose MWCO filters.

Filter MWCO Target MW
3 kDa 6 kDa < MW < 30 kDa
10 kDa 30 kDa < MW < 100 kDa
30 kDa 100 kDa < MW < 200 kDa
50 kDa 200 kDa < MW < 500 kDa
100 kDa 500 kDa < MW

 
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  • Cobetter_4mL_Centrifugal_Filter_User_Manual_20260129 Download
Which centrifuges are Cobetter centrifugal filters compatible with?

Briscale UF-15 centrifugal filters are compatible with centrifuges that accept 50 mL conical centrifuge tubes.

Briscale UF-4 centrifugal filters are compatible with centrifuges that accept 15 mL conical centrifuge tubes.

Both are suitable for horizontal rotors and fixed-angle rotors.

How to choose the appropriate centrifugal filters?

When selecting a centrifugal filter, the main considerations are the molecular weight of the target molecule and the desired concentration volume. For selecting the membrane cut-off, a general guideline is to choose a cut-off no greater than one-third of the target protein’s molecular weight.

For example, if the target protein has a molecular weight of 35 kDa, an ultrafiltration tube with a 10 kDa cut-off would be appropriate.

How should centrifugal filters be cleaned and stored?

Cleaning:
Soak the tube in 0.1–0.5 N NaOH solution for 30–60 minutes. Discard the NaOH solution, then centrifuge with ultrapure or deionized water for 10 minutes, repeating 1–2 times.

Storage:
Fill the tube with 0.05–0.1 N NaOH solution and store immersed.

Are Cobetter centrifugal filter single-use? Can they be reused?

Cobetter Briscale UF ultrafiltration centrifugal filters are single-use consumables.
For the same feed material, they can be reused 6–10 times with 30 min centrifugation per use without issues.

For different feed materials, it is recommended to use a new centrifugal filter.

Can Cobetter centrifugal filters be sterilized?

Cobetter Briscale UF ultrafiltration centrifugal filters are non-sterile and cannot be autoclaved. They can be disinfected using 0.1–0.5 N NaOH or 70% ethanol.

What cause low recovery rate after ultrafiltration?

There are four possible reasons:

1. Inappropriate ultrafiltration cut-off leading to product loss: Try using a smaller cut-off, generally following the 1/3 rule. For example, for a 10 kDa target molecule, the preferred cut-off is 3 kDa, while also testing 10 kDa and 30 kDa tubes to compare recovery.

2. Excessive concentration factor leading to incomplete recovery: Consider reducing centrifugation time and controlling the sample load.

3. Product integrity failure: Compare two centrifugal filters using buffer or pure water. A damaged centrifugal filter typically shows faster flow and lower concentrated volume.

4. Adsorption effects: If the initial sample concentration is below 0.1 mg/mL, adsorption inside the tube may reduce recovery.

Why does protein precipitation occur during concentration?

Protein precipitation may occur when the protein is concentrated too rapidly or to an excessively high concentration. Recommended optimization strategies:

1. Reduce the loading volume or centrifugation time to lower the final protein concentration.

2. Decrease the centrifugal force or centrifugation speed.

3. Increase mixing steps, breaking a single long centrifugation into multiple shorter centrifugation cycles.