Mini Hollow Fiber Filter Set with Bag TFF Ultrafiltration, Gamma Sterilized 1/pk
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Designed for single use, eliminating the need for post-use ClP cleaning, regeneration, and cleaning validation. This significantly saves operational time and avoids the risk of cross-contamination.
Pre-treated to reduce TOC and conductivity levels, effectively minimizing endotoxin and bioburden. No need for pre-use alkaline washing to remove pyrogens, enabling rapid experimental setup. Each filter is individually packaged and gamma-irradiated before shipment to ensure sterility.
Specifications
| Materials | Feed/Retentate connectors | Female Luer Lock | ||
| Permeate connector | Female Luer lock | |||
| Membrane material | mPES | |||
| Housing | Polysulfone | |||
| End Cap |
White-Polysulfone | |||
| Seals (internal) | Epoxy Resin | |||
| Physical Properties | Fiber ID |
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| No. of Fibers |
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| Effective Length |
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| OD*L (mm*mm) | 30cm: 10*346 60cm: 10*646 |
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| Effective Area |
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| Process Volume |
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| Flow Rate@2000s-1 (mL/min) |
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| Flow Rate@6000s-1 (mL/min) |
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| Sterility | Delivery Condition |
Sterile (Single use) | ||
| Sterilization Method | Gamma Irradiation at 25-45 kGy | |||
| Package | Pack Size | 1 pc/pk | ||
| Packaging | Individually packed in PE bag |
Features
- Pre-sterilized with gamma irradiation at an intensity of 25-45 kGy
- Pre-treated to reduce conductivity and TOC residue, minimizing wetting and shortening installation time
- Single-use design eliminates the need for post-use ClP cleaning
Applications
- Yeast, oncolytic bacteria, and other cell harvesting/purification
- Continuous cell perfusion culture
- Aseptic concentration of plasmids and mRNA in CGT applications
- Aseptic purification of recombinant proteins
Hollow Fiber Filter Configurations Compared
Sterilization Compatibility Comparison
Choose Fiber ID
- Module with fiber of 0.5mm ID is widely used in most application scenarios to improve mass transfer efficiency.
- Module with fiber of 1.0mm ID is ideal for products with high cell density or high solid content or high viscosity.
- Only module with fiber of 1.0mm ID is autoclavable, as steam cannot enter the fiber of 0.5mm ID effectively.
Share Rate
- The circulating flow rate of the hollow fiber depends on the product tolerance to the shear rate, in generally it will be set at 2000/s to 10000/s, which is much smaller than the shear rate generated by the turbulent flow on the surface of the cassette screen.
- For general materials, we usually choose a shear rate of 4000/s to 6000/s;
- If the product is sensitive to shear force (such as lentivirus, coronavirus or macromolecular protein expressed by animal cells, large plasmids and LNP, etc.), the shear rate needs to be reduced to 2000/s;
- If the product is with good resistance to shear force (such as small molecular proteins expressed by bacteria, etc.) shear rate could be increased to 8000/s to 10000/s.
How to choose hollow fiber MWCO?
Sufficient separation efficiency and the risks of fouling should both be taken into consideration when choosing MWCO.
To minimize the risk of fouling that inevitably occurs during membrane processing, it is advisable to choose membranes with relatively smaller pore sizes, as long as the separation efficiency and flux are maintained. This helps reduce the intrusion of impurity particles into the membrane pores, thus extending the membrane's service life.
Common processing scenarios are as follows:
- Heat source removal of small molecule in Chinese medicines: 5kD, 10kD
- Ultrafiltration and concentration of mABs, buffer exchange: 30kD, 50kD
- Concentration, purification and removal of virus: 100kD, 300kD, 500kD, 750kD
- Clarification of recombinant protein, antibody: 500kD, 750kD
- Concentration for bacteria: 500kD, 750kD
- Clarification of lysate: 0.2μm, 0.45μm, 0.65μm
Effective Length and Scale-Up
The process scale-up feature of hollow fibers is that: direct process scale-up can be carried out as long as the effective length is kept the same.
On the opposite, due to the significant pressure drop difference between inlet and outlet existed in different lengths, the internal pressure and flow velocity distribution of the flow channel also change correspondingly, so linear scale-up can not be performed on devices with different length. When processing with more fouling and high viscosity product, it is preferred to choose module with shorter length.
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