eShop » Laboratory » PVDF Membrane Western Blot » 0.2μm PVDF Transfer Membranes for Western Blot

0.2μm PVDF Transfer Membranes for Western Blot

In stock
Part No. 3550YH-R2703
$194.00 USD
Pore Size
Size
Package
Loading locations...
*Ships out within 3-7 Business Days
*Customers are responsible for any import duties or taxes
*Read our Shipping Policy and Refund Policy
*Not for medical use

Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The 0.45μm is suitable for most Western blotting applications and 0.2μm for immunoblotting of low MW proteins <20kDa.

Features

  • Smooth and flat surface, won't curl or fracture when cut
  • High mechanical strength, easy to be stripped and reprobed multiple times
  • Uniform pore size, high protein mobility, clear and neat bands
  • High sensitivity and low background, guarantee the success rate of low abundance protein detection

Applications

  • Protein transfer
  • Blot detection
  • Adsorption analysis

Quality Standards

  • ISO 9001:2015 Quality Management System
  • COA/COQ provided with shipment

Related Articles

  1. PVDF Transfer Membranes in Western Blot: Case Studies in SCI Journals
  2. PVDF vs Nitrocellulose Membrane in Western Blot – Which One to Choose?
  3. Why PVDF Transfer Membranes Are Ideal for Western Blotting Applications
Physical Properties Intended Use for Research Use Only
Filter Format Rolls, Sheets
Membrane Color White Plain
Dimentions 27.5 cm x 3.75 m /roll

8.4 x 7 cm /pc

15 x 15 cm /pc

20 x 20 cm /pc
Pore Size 0.22 μm
Wettability Hydrophobic
Materials Membrane Polyvinylidene Fluoride (PVDF)
Sterility Delivery Condition Non sterile
Sterilization Method N/A
Package Pack Size Pack of 1 roll

Pack of 50 pcs
Packaging in Carton

 

PVDF Transfer Membrane Performance

 
  • Cobetter_Laboratory_Consumables__Catalog__20260509 Download
  • MSDS_of_Cobetter_PVDF_Transfer_Membrane Download
  • Cobetter_PVDF_Transfer_Membrane_User_Manual_202603 Download
  • Cobetter_PVDF_Membrane_WesternBlot_Flyer_20260512 Download
  • Application_Note_Cobetter_PVDF_Transfer_Membrane_Western_Blot_Data_in_Biological_Literature Download
Does Cobetter PVDF transfer membrane have a front and back side? Does it require activation before use?

Cobetter PVDF transfer membranes have a smooth and uniform surface and do not require distinguishing between the front and back sides during use.

Before use, simply pre-wet the membrane with absolute ethanol or absolute methanol. After activation, the membrane changes from white and opaque to semi-transparent. A soaking time of 30–60 seconds is recommended.

Are Cobetter PVDF transfer membranes available in pre-cut sizes?

Cobetter transfer membranes are available both in roll format and as boxed, pre-cut sheets, to meet different experimental needs.

Roll format: 26.5 cm × 3.75 m
Pre-cut sheets: 200 mm × 200 mm; 84 mm × 70 mm

How to choose the appropriate pore size of PVDF transfer membranes?

Select the appropriate transfer membrane based on the molecular weight of the protein:

Small proteins (<20 kDa): use a 0.2 µm PVDF transfer membrane
Large proteins (>20 kDa): use a 0.45 µm PVDF transfer membrane

What should be considered when performing Western blotting for a high–molecular-weight protein (~200 kDa)?

Use a lower-percentage resolving gel, preferably around 7%.
Handle the gel carefully during stripping/removal to avoid damage.
Extend the transfer time to ensure efficient protein transfer.
Include a molecular weight marker, otherwise nonspecific bands may be difficult to interpret.

How should transfer conditions be set for Western blotting?

For proteins in the 15–100 kDa range, a typical condition is 300 mA constant current for 45 minutes.

For proteins >100 kDa, lower current (around 220 mA) is generally recommended with a longer transfer time.

Based on experience: ZO-1 (250 kDa) can be successfully transferred in 2.5 hours. 150 kDa proteins can be transferred in 1.5 hours.

For example: If the largest target protein is 200 kDa, the transfer condition can be estimated as: 220 mA × (200 / 150) ≈ 134 minutes, plus an additional ~15–20 minutes, resulting in a total transfer time of approximately 150 minutes.

How can background on the PVDF western blot membrane be reduced?

1. Ensure proper blocking. PVDF membranes require more stringent blocking steps. This can be achieved by increasing the concentration of the blocking agent 2-5 fold, increasing the blocking time, nd performing the procedure at 37 degrees C.

2. Optimizing the dilution ratios of the primary and secondary antibodies can reduce nonspecific background.

3. Perform thorough washing, and keep the membrane continuously wet and free of contamination during all steps.

What are the advantages of Cobetter PVDF transfer membranes?

1. Smooth and flat surface, resistant to curling.
2. High mechanical strength, allowing easy stripping and repeated use.
3. Uniform and consistent pore size, enabling high protein transfer efficiency and sharp, well-defined bands.
4. Higher sensitivity, improving the detection success rate of low-abundance proteins.

How should cellulose filter paper be selected for western blot?

The filter paper size should be slightly larger than the gel and the PVDF transfer membrane.

Cobetter pre-cut cellulose filter papers are designed to match the PVDF transfer membranes, offering convenient handling and an optimal fit.

1. NPF-OBP5 regulate sugar sensitivity and feeding behavior of rice planthoppers | Ruichuan Duan, et al. (2026) Insect Biochem Mol Biol. 188:104495. PMID: 41534801

2. Thermoelectric Bionic Skin Promotes Diabetic Wound Healing by Restoring Bioelectric Field Microenvironment | Dan Zhao, et al. (2025). Advanced Functional Materials.

3. SSBP1 promotes bovine ephemeral fever virus replication by antagonizing antiviral immune responses via degrading MAVS | Xingyu Li, et al. (2025). Vet Microbiol. 309:110684. PMID: 40848354

4. Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response | Jiayi He, et al. (2025). Ecotoxicol Environ Saf. 303:119006. PMID: 40939310

5. RNF126 suppresses amino acid-mediated mTORC1 signaling pathway by ubiquitinating ILF3 in HEK293T cells | Bin Hu, et al. (2025). Cell Signal. 112110. PMID: 40907628

6. VAPA suppresses BEFV and VSV-induced type I IFNs signaling response by targeting JAK1 for NEDD4-mediated ubiquitin-proteasome degradation | Tianhua Chen, et al. (2025). Vet Microbiol. 304:110456. PMID: 40080976

7. Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus | Yansi Xian, et al. (2025). Bone Res. 13(1):30. PMID: 40025004

8. MARCH1 negatively regulates TBK1-mTOR signaling pathway by ubiquitinating TBK1 | Xiao Li, et al. (2024). BMC Cancer. 24(1):902. PMID: 39061024