Home » Process Filtration » Minilab TFF System » Ultrafiltration Cassettes » Consieve Tangential Flow Filtration Cassette PES Ultrafiltration Membrane Lab, 1/pk
Lab TFF Cassette PES on white background
Lab TFF Cassette PES 0.11m² white background
Lab TFF Cassette PES on white background
Lab TFF Cassette PES 0.11m² white background

Consieve Tangential Flow Filtration Cassette PES Ultrafiltration Membrane Lab, 1/pk

In stock
Part No. UFELA0001001P
$256.00 USD
Filtration Area
MWCO
Screen Type
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*Not for medical use

Consieve™ UET tangential flow filtration cassette is made of asymmetric mPES (modified Polyethersulfone) membrane, which provides high flow rate, low protein bindings and wide pH compatibility. The cassette is easy to clean, install and has a high retention efficiency, with low working volume and high efficiency which ensures product yields.

Consieve™ UET ultrafiltration TFF cassettes are available in a wide range of molecular weight cut-offs from 1 kDa to 1000 kDa. It can be easily scale-up from Lab, Pilot to Process scale with a wide volume range from 0.5L to 20,000L. They are widely used in separation, concentration and diafiltration in downstream bioprocessing. 

The cassettes should be used with Cobetter Stainless Steel TFF Cassette Holder:

EFA 0.01m² or 0.02m² cassettes use with Ultrafiltration Cassette Holders (0.01m²)

EFA 0.02m² or 0.11m² cassettes use with Ultrafiltration Cassette Holders (0.11m²)

EFA 0.5m² or 2.5m² cassettes use with Ultrafiltration Cassette Holders (0.5m²)

Features

  • High flow rate
  • Low protein binding
  • High product yield
  • No glue used, resulting in low extractables
  • pH range: 1-14

Applications

  • Concentration and desalting of proteins, peptides or nucleic acids
  • Buffer exchange
  • Fractionation and purification
  • Sample preparation before chromatography
  • Remove pyrogens from water, buffers, media solutions
Materials Membrane material mPES
Screen PP
Seals(internal) Silicone
Encapsulant White polyurethane Silicone
Gaskets Platinum cured silicone
Physical Properties MWCO 1/3/5/8/10/30/50/100/300/500/1000 kDa
Pressure Drop 0.3-0.6 bar (Circulation Flow Rate 6L/min/m²)
Effective Filtration Area
0.01 m² (0.11 ft²) 0.11 m² (1.2 ft²)
Throughput
200-2000mL 5-50L
Dead Volume
2mL 12mL
Diffusion Flow @0.1Mpa
≤1.1 mL/min ≤12 mL/min
Corssflow Rate @0.2Mpa
77-154 mL/min   0.84-1.68 L/min
Operating Conditions Max. Operating Temperature 50°C
Max. Operating Pressure 4.0 bar
pH Range 1.0-14.0
Sterility Delivery Condition
 Nonsterile
Package Packaging Individually packed in PE bag
Pack Size 1 pc/pk


Screen Difference

Screen Type Flow Resistance Flow Rate (LMM@dp 15psi Water) Applications
Tight Screen High 5~6 Generates the strongest turbulence on the membrane surface. Tight Screen can achieve relatively high tangential flow velocity at a relatively low inlet flow rate, but has a high pressure drop (dp) and is easily clogged by particles. Tight screen cassettes are suitable for samples without particles, low viscosity, and low concentration.
Standard Coarse Screen Medium 8~11 Generates moderate turbulence on the membrane surface, reducing clogging. Standard Coarse Screen requires a higher inlet flow rate to match the appropriate tangential flow velocity. The pressure drop (dp) is significantly lower compared to Tight Screens. Standard coarse screen cassettes are suitable for clarified samples with medium to high viscosity.
Suspended Screen Low ≥40 Generates the weakest turbulence and low shear force on the surface, but still significantly reduces clogging. Theoretically, Suspended Screen requires a very high inlet flow rate. Due to its open screen structure, it can accommodate more particles (cells, cell debris) without clogging. Suspended screen cassettes are suitable for unclarified samples with high particle content or high viscosity, and samples that are sensitive to shear force.

 
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What is tangential flow filtration (TFF)?

Tangential flow filtration (TFF) is a method in which the feed flows along the membrane surface, generating tangential shear to reduce membrane fouling and clogging. TFF is commonly applied as ultrafiltration (1–1000 kDa) or microfiltration (0.1–0.65 µm).

How to choose the appropriate ultrafiltration membranes?

For most ultrafiltration and dialysis applications, both PES and RC membranes are suitable choices.

PES membranes can meet a wide range of application needs for most protein and small-molecule products, such as antibodies, APIs, and vaccines.

RC membranes should be considered when preparing high-concentration formulations, applications containing organic solvents (e.g., ADC products), or concentration/exchange of linear molecules including oligonucleotides and peptides.

For new target molecules, it is recommended to test both membrane types during standard sample handling and process optimization.

How to select the appropriate cut-off for ultrafiltration cassettes?

Choose the membrane cut-off based on the size or molecular weight of the substance to be separated or retained:

Antibody proteins: 30 kDa or 50 kDa
Viral or vaccine products: 100–1000 kDa
For API products, an appropriate molecular weight cut-off (MWCO) can be selected from the range of 1 kDa, 2 kDa, 3 kDa, 5 kDa, 8 kDa, and 10 kDa according to the process objectives.

If the molecular weight of the target substance is unknown, preliminary testing can be performed using ultrafiltration centrifugal devices with different cut-offs to identify the most suitable molecular weight retention.

What materials and cut-offs are available for Cobetter TFF cassettes?

Ultrafiltration cassettes are available in PES and RC membranes, with cut-offs ranging from 1–1000 kDa.

Microfiltration cassettes are available in PES and PVDF membranes, with pore sizes ranging from 0.1–0.65 µm.

Can TFF cassettes be reused?

Yes. TFF cassettes can be reused by selecting appropriate cleaning methods. However, due to changes such as declining membrane flux and increased permeation, the cassettes should be replaced periodically as needed.

How should TFF cassettes be cleaned?

They can be cleaned by circulating 0.1-0.5 M sodium hydroxide (NaOH) solution. If cleaning effectiveness is insufficient, adjustments can be made by either of the following methods:

  • Increasing the cleaning solution temperature (<45 °C)
  • Adding sodium hypochlorite (NaClO, 500 ppm)
  • Extending the cleaning time

For difficult-to-clean feed components, alternative cleaning solutions such as acidic solutions, alcohols, or surfactants may also be used.

Are Cobetter TFF cassettes compatible with hardware from other companies?

Yes. They can be installed in holders from Merck, Pall, and similar manufacturers. But it is necessary to ensure proper torque and perform integrity testing.

For Sartorius holders, a specific adapter plate is required for proper installation.