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Oro prep™ ELISpot 96-Well Plate Hydrophobic PVDF Membrane

In stock
Part No. OP96EAPVGXP
$657.00 USD
Pore Size
Sterility
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*Not for medical use

ELISpot, as a highly sensitive key tool in cellular immunology, is widely applied in cutting edge fields such as vaccine development, immunogenicity evaluation, and drug screening.

Cobetter Oro prep™ ELISpot 96 well plates are non pre-coated antibody type, consisting of a highly adaptable 96 well plate integrated with a high performance PVDF membrane. The product includes an upper cover, transparent 8 tube strips with PVDF membranes attached to the bottom and a base.

Features

Hydrophobic PVDF Membrane

  • Low background, suitable for detection of most cytokines
  • Stable and uniform membrane with excellent protein adsorption capacity

Plate Frame Design

  • Compatible dimensions with common plate readers on the market, enhancing reading efficiency
  • Made of high-transparency PMMA material, facilitating microscopic observation
  • Secure bonding and sealing between the plate frame and membrane, ensuring no leakage

Sterility

  • Manufactured in a Class 100,000 cleanroom environment with sterile certification, minimizing the risk of cell culture contamination

Quality Standards

  • ISO 9001:2015 Quality Management System
  • Manufactured for use in conformance with cGMP
  • COA/COQ provided with shipment
  • Endotoxin <0.25 EU/mL (LAL test, Aqueous extraction), USP<85>
Materials Membrane 0.45 μm Hydrophobic PVDF
Plate Material

Plate: PMMA

Lid: PS
Physical Properties Binding Type High binding surface
Wells Flat bottom wells
Size

Length: 127.8 mm

Width: 85.5 mm

Height without lid: 14.4 mm
Well Filtration Area 0.28 cm²
Well Maximum Volume 300 μL
Working Volume 50-250 μL
Sterility Sterilization Method Non-sterile
gamma-compatible
Package Pack Size Pack of 10
Packaging Individually packed in a double PE bag

 
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What is the sterilization method for Cobetter multi-well filter plates? And what is the shelf life?

Cobetter multi-well filter plates are available in both non-sterile and sterile.
Sterilization method is by 25-45 kGy gamma irradiation.
The shelf life of nonsterile plate is 3 years, and that of sterile plate is 2 years.

How to ensure the multi-well filter plate has no leakage? And can integrity testing be performed?

Cobetter multi-well filter plates undergo pressure-hold testing using dedicated equipment before shipment.

When tested at pressures below the bubble point, no premature bubble formation is observed, confirming no leakage and ensuring the product meets release criteria for shipment.

What is the maximum loading volume for Cobette multi-well filter plate?

Cobetter provides both 24-well and 96-well filter plates. The recommended loading volume for the 24-well filter plate is 7 mL, and for the 96-well filter plate it is 300 µL.

How to use the multi-well filter plate?

Cobetter multi-well filter plate is compatible with both centrifugal filtration and vacuum filtration.

Recommended operating conditions: for the 24-well plate, use a vacuum level of 10 inHg or a centrifugal force of 1000 G; for the 96-well plate, use a vacuum level of 10 inHg or a centrifugal force of 1000 G.

If the material is difficult to filter, the vacuum level or centrifugal force can be appropriately increased.

Can Cobetter multi-well plates be compatible with automation equipment?

Cobetter Multi-well plates follow the ANSI/SBS standards, which ensures globally uniform specifications for seamless compatibility and efficient integration with automated equipment and laboratory instruments.

What are the advantages of Cobetter multi-well filter plate?

Cobetter multi-well filter plates feature low extractables, low adsorption, and high throughput. In addition, we can offer fully customized services based on material requirements and different process needs.

What materials are available for Cobetter multi-well filter plates?

Cobetter multi-well filter plates are available in a wide range of materials, including PES, PVDF, Nylon, PTFE, PP, glass fiber, and depth filter media.

Customized configurations are also available, such as depth filter media combined with PES membranes or glass fiber combined with PES membranes, to meet specific application requirements.

Can Cobetter multi-well filter plate be reused?

Cobetter multi-well filter plates are disposable consumables, and are not recommended for reuse.