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0.5mm Lab Hollow Fiber TFF mPES Ultrafiltration Membrane
Membrane PESMWCO 3/5/10/30/50/100/300/500/750kDaFiltration Area 236cm²/ 471cm²Package 1/pk -
1mm Lab Hollow Fiber TFF mPES Ultrafiltration & Microfiltration Membrane
Membrane PESMWCO 3kDa~0.65μmFiltration Area 170cm²/ 340cm²Package 1/pk -
1mm Lab Hollow Fiber TFF mPES Membrane, Autoclavable
Membrane PESMWCO 750kDa/0.2μmFiltration Area 170cm²/ 340cm²Package 1/pk -
0.5mm Lab+ Hollow Fiber TFF mPES Ultrafiltration Membrane
Membrane PESMWCO 3/5/10/30/50/100/300/500/750kDaFiltration Area 0.08m²/ 0.16m²Package 1/pk
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Hollow Fiber Filters
Cobetter hollow fiber filters are made of low-binding modified polyethersulfone membranes (m-PES) that deliver excellent filtration performance. The low shear stress makes it ideal for the processes of cell harvest, clarification, diafiltration and concentration where gentle and fast separation is needed. They are easy to install, clean and sanitize, providing cost-efficient and time-saving solutions for your bioprocessing applications. Cobetter hollow fiber filter modules are offered with a broad range of sizes and surface areas as Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.
Overview
Coetter hollow fiber filters are available with a broad range of sizes for easy scale-up from small volume R&D applications, small volume pilot to commercial production.
Features
· A broad range of sizes for easy scale-up
· Hydrophilic modified PES membrane provides low protein binding
· Cost-efficient and time saving
· Quick installation and easy operation, no holder required
Applications
· Purification, concentration and diafiltration of viral vector
· Clarification of cells and bacterial from fermentation broth
· Clarification of cell lysates or cell debris
· Concentration and diafiltration of proteins
· Perfusion applications
How to use a hollow fiber membrane filter?
Module Preparation
For lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.
Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.
- Rinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L/m².
- Drain module and perform module integrity test.
Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.
Module Integrity Test
All hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.
- Diffusion Flow Method
- Pressure Retaining Method
Processing Buffer Equilibrium
The purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.
Cleaning after Use
The hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.
Hollow Fiber Storage
The hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4°C.
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The Applications of Hollow Fibers in Upstream Bioprocessing
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